IngentaConnect Novelcross-Linking Agents for Hydrogelation of Biological Polymer...

Authors: Miyamoto K.; Tokita M.; Komai T.

Source: Protein and Peptide Letters, Volume 8, Number 3, June 2001 , pp. 231-236(6)

Publisher: Bentham Science Publishers

Abstract:

Cross-linking agents are useful for investigating the biological functions of biological polymers. Here we would like to report the preparation of a 4-hydroxyphenyldimethyl-sulfoniummethlysulfate (DSP) activated cross-linker that can cross-link proteins as a novel cross-linking agents. By using this cross-linker, gelatin can be cross-linked both in the sol-state at 40 degree centigrade and in the gel-state at 2 degree centigrade. The chemically cross-linked gelatin gels by DSP-activated esters that were obtained in gel-state and sol-state at a gelatin concentration greater than 1percent and 5percent, respectively. The fixed conformation of the folding structure seen with this physical cross-linking system is thought to result from the structure of the native protein. In addition, the urea concentration dependence of the swelling ration indicates that the hydrogen bonds are much more stable in the gel prepared from the gel state of gelatin than those in the gel prepared from the sol state of gelatin. As result, these novel bifunctional agents of the hydrogelation of biological polymers are expected application in studying intermolecular interaction.

Keywords: Cross Linking Agents; Hydrogelation; amino acid; gelatin

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409481

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Novelcross-Linking Agents for Hydrogelation of Biological Polymers