Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris
Abstract:Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.
Document Type: Review Article
Publication date: 2001-06-01
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