Functional Expression of a Galactose-Binding C-Type Lectin In Pichia pastoris

Authors: Li J-d.; Xu Q.; Wang K-y.

Source: Protein and Peptide Letters, Volume 8, Number 3, June 2001 , pp. 217-224(8)

Publisher: Bentham Science Publishers

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Abstract:

Trimeresurus stejnegeri lectin (TSL) was expressed in the yeast Pichia pastoris under the control of alcohol oxidase (AOX1) promoter. The recombinant protein, which was glycosylated, can be purified by one-step affinity chromatography. The purified recombinant TSL shared similar sugar-binding activity and Specificity with the native protein as determined by hemagglutination and enzyme-linked lectin binding Assays.

Keywords: C-TYPE LECTIN; PICHIA PASTORIS; Trimeresurus stejnegeri lectin (TSL); alcohol oxidase (AOX1) promoter; galactose-binding C-type; mutagenesis; alcohol oxidase (AOX1)

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409463

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