Cloning and Expression of a Novel Long Neurotoxin from Bungarus multicinctus in E. coli

Authors: Cai Q.; Yang S-l.; Gong Y.

Source: Protein and Peptide Letters, Volume 8, Number 3, June 2001 , pp. 201-207(7)

Publisher: Bentham Science Publishers

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Abstract:

The total RNA was isolated from Bungarus multicinctus venom. A novel long neurotoxin (LNT) cDNA was amplified by RT-PCR method. Sequence analysis revealed that the cDNA of long neurotoxin has an open reading frame encoding 94 amino acids, including a signal peptide of 21 amino acids. The mature peptide encoding sequence was inserted into plasmid pET-28 (a+), and then which was expressed in the E. coli BL21 (DE3). The expressed Protein is identified as a novel long neurotoxin by western blot.

Keywords: NEUROTOXIN; total RNA; neurotoxin (LNT) cDNA

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409508

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