Cloning and Expression of a Novel Long Neurotoxin from Bungarus multicinctus in E. coli

Authors: Cai Q.; Yang S-l.; Gong Y.

Source: Protein and Peptide Letters, Volume 8, Number 3, June 2001 , pp. 201-207(7)

Publisher: Bentham Science Publishers

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Abstract:

The total RNA was isolated from Bungarus multicinctus venom. A novel long neurotoxin (LNT) cDNA was amplified by RT-PCR method. Sequence analysis revealed that the cDNA of long neurotoxin has an open reading frame encoding 94 amino acids, including a signal peptide of 21 amino acids. The mature peptide encoding sequence was inserted into plasmid pET-28 (a+), and then which was expressed in the E. coli BL21 (DE3). The expressed Protein is identified as a novel long neurotoxin by western blot.

Keywords: NEUROTOXIN; total RNA; neurotoxin (LNT) cDNA

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866013409508

Publication date: 2001-06-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.