Purification and Identification of A 25 KDa Hemorrhagin from B. atrox Venom

Authors: Petretski J.H.; Kanashiro M.M.; Rodriques F.G.; Alves E.W.; Machado O.L.T.; Kipnis T.L.

Source: Protein and Peptide Letters, Volume 8, Number 3, June 2001 , pp. 187-192(6)

Publisher: Bentham Science Publishers

Abstract:

Viperine and crotaline snake venoms contain one or more hemorrhagic metalloproteinases called hemorrhagins. The most potent hemorrhagins belong to P-III class and have, in adition to the protease domain, a disintegrin-like and a cysteine-rich domains. Although proteolytic degradation of vascular endothelium basement membrane has been established to be the main factor responsible for hemorrhage, several studies reveal other factors that actually do facilitate this process. In this study we report the identification of an P-I class hemorrhagin from Bothrops atrox venom. We have formerly purified an P-III class hemorrhagin from the same venom. Although exhibiting a higher proteolytic activity, the P-I class hemorrhagin showed to be a less efficient hemorrhagin when compared in vivo with the previously described P-III class metalloprotease.

Keywords: HEMORRHAGIN; hemorrhagins; P-III class; Bothrops atrox; P-III class metalloprotease

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866013409535

Publication date: 2001-06-01

• Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.

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• In this Subject: Anatomy & Physiology
• By this author: Petretski J.H. ;  Kanashiro M.M. ;  Rodriques F.G. ;  Alves E.W. ;  Machado O.L.T. ;  Kipnis T.L.

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