Expression of Recombinant Hepatitis B Virus Antigen Hbsag in Transgenic Plant Callus

Authors: Kawashima C.G.; Baba E.H.; Hansen E.

Source: Protein and Peptide Letters, Volume 8, Number 2, April 2001 , pp. 97-100(4)

Publisher: Bentham Science Publishers

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Abstract:

The coding sequence of the surface antigen HBsAg of hepatitis B virus was cloned into a plant expression vector under the control of cauliflower mosaic virus 35S promoter. Cotyledons, hypocotyls and roots of lettuce cultivar Vit?ria were transformed by cocultivation with Agrobacterium tumefaciens harbouring the recombinant plasmid. Lettuce callus tissue grown on selective medium was analysed and the presence of the transgene confirmed by PCR. Expression of recombinant protein HbsAg was confirmed by Western Blot.

Keywords: hepatitis b virus antigen hbsag; agrobacterium tumefaciens; hepatitis b virusantigen HBsAg; polypeptides; antigenicity; immunogenicity

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866013409571

Publication date: 2001-04-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.