Crystallization And Preliminary X-Ray Diffraction Study Of Riboflavin Synthase

Authors: Wawrazak Z.; Viitanen P.V.; Calabrese J.C.; Jordan D.B.

Source: Protein and Peptide Letters, Volume 8, Number 1, February 2001 , pp. 69-73(5)

Publisher: Bentham Science Publishers

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Abstract:

Escherichia coli riboflavin synthase crystallizes at 22 C in the presence of 7-10percent by volume diglyme, 20-50 mM MgCl2 and pH 7.0. In this medium diffraction quality crystals are routinely obtained within 5 h and they are stable for 10 weeks. The crystals are orthogonal in space group P212121 with unit cell dimensions of a=52.4 A , b = 62.1 A, c = 218.8 A. A 97percent complete data set was collected at 2.1 A resolution.

Keywords: RIBOFLAVIN SYNTHASE

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409698

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