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Real-Time Polymerase Chain Reaction Detection Methods

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Abstract:

Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification. Real-time assay chemistries are subdivided into several main categories including DNA-binding agents, molecular beacons, hybridization probes, hydrolysis probes, and dye-primer based systems. Specific advantages and applications of each category are highlighted herein.





Keywords: FRET probes; Kinetic PCR; Quantitative PCR; Real-time PCR; SYBR® Green; TaqMan®; hairpin probes; hybridization probes; hydrolysis probes; molecular beacon

Document Type: Research Article

DOI: https://doi.org/10.2174/187221508783406558

Publication date: 2008-01-01

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  • Recent Patents on DNA and Gene Sequences publishes review articles by experts on recent patents on DNA and gene sequences. A selection of important and recent patents in the field is also included in the journal. The journal is essential reading for all researchers involved in applied molecular biology.
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