Skip to main content

Real-Time Polymerase Chain Reaction Detection Methods

Buy Article:

$63.00 plus tax (Refund Policy)

Real-time Polymerase Chain Reaction (PCR) is a quickly developing technology that has built upon the classic end-point PCR detection methods. In this article, we will review recent patents related to various chemistries used for nucleic acid detection during real-time PCR amplification. Real-time assay chemistries are subdivided into several main categories including DNA-binding agents, molecular beacons, hybridization probes, hydrolysis probes, and dye-primer based systems. Specific advantages and applications of each category are highlighted herein.

No References
No Citations
No Supplementary Data
No Article Media
No Metrics

Keywords: FRET probes; Kinetic PCR; Quantitative PCR; Real-time PCR; SYBR® Green; TaqMan®; hairpin probes; hybridization probes; hydrolysis probes; molecular beacon

Document Type: Research Article

Publication date: 01 January 2008

More about this publication?
  • Recent Patents on DNA and Gene Sequences publishes review articles by experts on recent patents on DNA and gene sequences. A selection of important and recent patents in the field is also included in the journal. The journal is essential reading for all researchers involved in applied molecular biology.
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more