Skip to main content

Ligands Turning Around in the Midst of Protein Conformers: The Origin of Ligand-Protein Mating. A NMR View

Buy Article:

$63.00 plus tax (Refund Policy)

Abstract:

Protein-ligand binding is a puzzling process. Many theories have been devised since the pioneering key-and-lock hypothesis based on the idea that both the protein and the ligand have a rigid single conformation.

Indeed, molecular motion is the essence of the universe. Consequently, not only proteins are characterized by an extraordinary conformational freedom, but ligands too can fluctuate in a rather vast conformational space. In this scenario, the quest to understand how do they match is fascinating.

Recognizing that the inherent dynamics of molecules is the key factor controlling the success of binding and, subsequently, of their chemical/biological function, here we present a view of this process from the NMR stand point.

A description of the most relevant NMR parameters that can provide insights, at atomic level, on the mechanisms of protein- ligand binding is provided in the final section.

Keywords: (R2) a; 15N; CRABPI; CRBP; CRBP type I; CaM-MLCK; Calmodulin; DHFR; Enzymes; HSQC; MUP I; MWC; NAMFIS; NH bonds; NMR; NMR spectroscopy; NOE; PCS; PDF; PRE; Paramagnetic Mapping; R1; RDCs; Rex; S2; SRLS; STD; X-ray crystallography became; bound/unbound states; cofactor; conformation; conformational selection model; cytidylyl-transferase en-zyme; enzyme 1; equilibrium; fine tuning; flexibility; h3JNC; hemophore; heterogeneity; ligand; ligand binding; nduced-fit model; per-turbation; probability distribution of states; protein; reactivity; rigidification; signal transduction; substrate; tr-NOE; ubiquitous protein

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/156802611794863535

Publication date: January 1, 2011

ben/ctmc/2011/00000011/00000002/art00004
dcterms_title,dcterms_description,pub_keyword
6
5
20
40
5

Access Key

Free Content
Free content
New Content
New content
Open Access Content
Open access content
Subscribed Content
Subscribed content
Free Trial Content
Free trial content
Cookie Policy
X
Cookie Policy
ingentaconnect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more