The High pH and pH-Shift Refolding Technology

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Abstract:

We have developed a high-pH, pH-shift refolding “Ph-Fold technology”, both for academic research and industrial protein drug development applications. Using this technology, we were able to refold some “difficult-to-refold” proteins, some of which are proven important drug targets and protein drug candidates. The technology is composed of the initial E. coli production of inclusion bodies, the high pH solubilization/pH shift refolding screening technology, which includes automated pH shift refolding screening, and the methods of testing protein refolding without functional assay. This technology, especially the automated refolding system based on the Ph-Fold technology, may help both academic and industrial research in broadening structural genomic research, functional proteomics, and genomic scale drug development efforts.





Keywords: E. coli expression; high pH reflolding; inclusion bodies; pH shift refolding

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/138920110791111906

Publication date: April 1, 2010

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  • Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.
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