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Urea-Gradient Protein Refolding in Size Exclusion Chromatography

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Abstract:

Protein refolding using urea gradient size exclusion chromatography (UGSEC) is a new version of conventional SEC refolding, which incorporates urea gradient into the SEC. Operating factors, mainly the urea gradient length and the final urea concentration in the gradient, are discussed in detail. Recent applications of UGSEC, including refolding of recombinant human granulocyte colony stimulating factor by UGSEC, is shown as an example.





Keywords: Urea gradient; protein refolding; recombinant human granulocyte colony stimulating factor; size exclusion chromatography

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/138920110791112004

Publication date: April 1, 2010

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  • Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.

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