Development of an Insect Cell-Free System

Authors: Ezure, Toru; Suzuki, Takashi; Shikata, Masamitsu; Ito, Masaaki; Ando, Eiji; Utsumi, Toshihiko; Nishimura, Osamu; Tsunasawa, Susumu

Source: Current Pharmaceutical Biotechnology, Volume 11, Number 3, April 2010 , pp. 279-284(6)

Publisher: Bentham Science Publishers

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Abstract:

Cell-free protein synthesis systems offer production of native proteins with high speed, even for the proteins that are toxic to cells. Among cell-free systems, the system derived from insect cells has the potential to carry out posttranslational modifications that are specific to eukaryotic organisms, as occurs in the rabbit reticulocyte system. In this review, we describe development of this insect cell-free system and its applications.

Keywords: Cell-free protein synthesis; 5'-UTR; Freeze-thawing; Insect cell; MALDI-TOF MS; Post-translational modification; Rabbit reticulocyte

Document Type: Research article

DOI: http://dx.doi.org/10.2174/138920110791111997

Publication date: 2010-04-01

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Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.