Skip to main content

The Wheat-Germ Cell-Free Expression System

Buy Article:

$55.00 plus tax (Refund Policy)

We have made a dramatic improvement of the wheat cell-free protein synthesis system. The first key improvement is the method for preparation of the cell-free extract that is free of inhibitory factors of translation reaction. Additional improvements include a method for preparation of transcription-ready templates by PCR, an expression vector for the cell-free system, and the “bilayer” mode reaction method that is much more efficient than the batch mode method and at the same time easy to be performed by human hands and by liquid handling machines. We review here the history of the development and describe the protocols for the most handy “bilayer” method and a more efficient but complicated methods. Information on many examples and variations of the wheat cell-free protein synthesis methods already published elsewhere is then provided so that the readers can understand the power and potential applications of the methods.





No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: Cell-free protein synthesis; automation; pEU; split-primer PCR; wheat

Document Type: Research Article

Publication date: 2010-04-01

More about this publication?
  • Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more