Fusion Partners as a Tool for the Expression of Difficult Proteins in Mammalian Cells

Authors: Zhang, Jue; Carter, Jane; Siu, Sophia; W. O'Neill, Jason; H. Gates, Andrew; Delaney, John; Mehlin, Christopher

Source: Current Pharmaceutical Biotechnology, Volume 11, Number 3, April 2010 , pp. 241-245(5)

Publisher: Bentham Science Publishers

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Abstract:

The expression of proteins which do not express well on their own can be enhanced by linking them to human serum albumin (HSA) or antibody crystallizable fragment (Fc). The constructs shown here are designed to secrete the proteins after transient transfection of mammalian cell lines. The fusion partners are appended to the N-terminus of the proteins and contain a linker designed to be proteolytically cleaved. Transient transfection and purification protocols are provided as well as experimental results with five interleukins.

Keywords: Fusion proteins; protein expression; transfection; 2936E cells; polyethyleneimine; PEI

Document Type: Research article

DOI: http://dx.doi.org/10.2174/138920110791111898

Publication date: 2010-04-01

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Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.