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Single-Molecule Detection and Probe Strategies for Rapid and Ultrasensitive Genomic Detection

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This paper reviews the current state-of-the-art development of single-molecule detection (SMD)-based methods for ultrasensitive and specific analysis of genomic sequences. We first discuss several newly devised single fluorescent probe strategies that allow separation-free detection of low-abundance DNA sequences, such as quantum dot (QD)- mediated fluorescence resonance energy transfer (FRET) technology and dual-color fluorescence coincidence and colocalization analysis. Various schemes toward single DNA sizing and sequencing in solutions or on surfaces are also reviewed. In the end, we summarize the different microfluidic approaches developed for use with SMD to facilitate rapid, low-volume and quantitative analysis, such as electrokinetic and hydrodynamic single-molecule manipulation techniques.





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Keywords: Dual-color fluorescence coincidence; Fluorescence resonance energy transfer; Microfluidics; Nucleic acid detection; Quantum dot; Single molecule detection

Document Type: Research Article

Affiliations: Department of Mechanical Engineering & Whitaker Biomedical Engineering Institute, The Johns Hopkins University, Baltimore, Maryland 21218, USA;

Publication date: 01 December 2005

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  • Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.
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