Authors: Grunwald, D.; Cardoso, M. C.; Leonhardt, H.; Buschmann, V.

Source: Current Pharmaceutical Biotechnology, Volume 6, Number 5, October 2005 , pp. 381-386(6)

Publisher: Bentham Science Publishers

Abstract:

During the last years, Fluorescence Correlation Spectroscopy (FCS) has proven to be a powerful tool for basic research in many applications. The combination of a minimal detection volume in the femtoliter range coupled with very high sensitivity extends the possibilities to design sensitive homogeneous tests. In this article we illustrate the analysis of binding processes with FCS based on the changes in diffusion characteristics of GFP upon binding to an antibody. Problems induced by highly heterogeneous samples are discussed and differences of GFP binding to a monoclonal and a polyclonal antibody are shown and analyzed. We stress data processing, limitations and useful approximations in FCS methodology. Basic ideas of data acquisition and processing as well as new developments and applications are presented.

Keywords: fluorescence correlation spectroscopy; gfp; kinetics; binding assay; antibody

Document Type: Review article

DOI: http://dx.doi.org/10.2174/138920105774370616

Affiliations: 1: Max-Delbruck-Center for Molecular Medicine, Wildtbergstr. 50, 13125 Berlin, Germany.

Publication date: 2005-10-01

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• Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.

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