Art and Artefacts of Fluorescence Correlation Spectroscopy

Authors: Jorg Enderlein; Ingo Gregor; Digambara Patra; Jorg Fitter

Source: Current Pharmaceutical Biotechnology, Volume 5, Number 2, April 2004 , pp. 155-161(7)

Publisher: Bentham Science Publishers

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Abstract:

Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient of the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.

Keywords: fluorescence correlation spectroscopy; diffusion coefficients; confocal detection

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1389201043377020

Affiliations: 1: Institute for Biological Information Processing 1 & 2, Forschungszentrum Julich, D-52425 Julich, Germany.

Publication date: 2004-04-01

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Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.