Art and Artefacts of Fluorescence Correlation Spectroscopy
Authors: Jorg Enderlein1; Ingo Gregor1; Digambara Patra1; Jorg Fitter1
Source: Current Pharmaceutical Biotechnology, Volume 5, Number 2, April 2004 , pp. 155-161(7)
Publisher: Bentham Science Publishers
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Abstract:
Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient of the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.Keywords: fluorescence correlation spectroscopy; diffusion coefficients; confocal detection
Document Type: Review article
DOI: 10.2174/1389201043377020
Affiliations: 1: Institute for Biological Information Processing 1 & 2, Forschungszentrum Julich, D-52425 Julich, Germany.
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