Art and Artefacts of Fluorescence Correlation Spectroscopy

Authors: Jorg Enderlein¹; Ingo Gregor¹; Digambara Patra¹; Jorg Fitter¹

Source: Current Pharmaceutical Biotechnology, Volume 5, Number 2, April 2004 , pp. 155-161(7)

Publisher: Bentham Science Publishers

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Abstract:

Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient of the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.

Keywords: fluorescence correlation spectroscopy; diffusion coefficients; confocal detection

Document Type: Review article

DOI: 10.2174/1389201043377020

Affiliations: 1: Institute for Biological Information Processing 1 & 2, Forschungszentrum Julich, D-52425 Julich, Germany.

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