The LSM 510 META - ConfoCor 2 System: An Integrated Imaging and Spectroscopic Platform for Single-Molecule Detection

Authors: Klaus Weisshart; Volker Jungel; Stephen J. Briddon

Source: Current Pharmaceutical Biotechnology, Volume 5, Number 2, April 2004 , pp. 135-154(20)

Publisher: Bentham Science Publishers

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Abstract:

Fluorescence Correlation Spectroscopy (FCS) has developed into a routine method to quantitatively study diffusion of molecules and kinetic processes. FCS has recently become popular to complement live cell imaging with biophysical information. The enabling technology has been commercially realised by combining laser scanning microscopes and fluorescence correlation spectrometers in one integrated platform. This article provides an overview of the Zeiss solution of such a combined instrument, the LSM 510 META / ConfoCor 2 system. We focus on the instrumental set up as well as technical advances and improvements in the software that controls FCS data acquisition and evaluation. In addition, we outline the calibration of the instrument and the work flow for data analysis with emphasis on in vivo pharmacological applications.

Keywords: LSM 510 META; spectrometers; ConfoCor 2 system

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1389201043376913

Affiliations: 1: Carl Zeiss Jena GmbH, Carl- Zeiss-Promenade 10, 07745 Jena, Germany.

Publication date: 2004-04-01

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Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.