The Cellular and Molecular Basis of Health Benefits of Grape Seed Proanthocyanidin Extract

Authors: Joshi S.S.; Kuszynski C.A.; Bagchi D.

Source: Current Pharmaceutical Biotechnology, Volume 2, Number 2, June 2001 , pp. 187-200(14)

Publisher: Bentham Science Publishers

Abstract:

Red grape seed extract containing proanthocyanidins and other antioxidants are being used as nutritional supplements by many health conscious individuals. The beneficial effects of grape seed proanthocyanidins (GSPE) have been reported, however, little is known about their mechanism(s) of action. One of the beneficial effects of GSPE is chemoprevention of cellular damage. The precise mechanism by which GSPE mediates, chemoprevention is not yet understood. This report addresses this issue. We investigated the mechanisms of actions of GSPE, which ameliorates chemotherapy-induced toxic effects of Idarubicin (Ida) and 4,-hydroxyperoxycyclophosphamide (4-HC) in normal human Chang liver cells. Exposure to GSPE resulted in a significant reduction in apoptosis in response to the cytotoxicity of chemotherapeutic agents. RT-PCR analysis showed a significant increase in the anti-apoptotic gene Bcl-2 and a decrease in the cell cycle associated and proapoptotic genes, c-myc and p53 in cells treated with GSPE. These results suggest that some of the chemopreventive effects of GSPE are mediated by upregulating Bcl-2 and down regulating c-myc and p53 genes.

Keywords: Grape Seed; Proanthocyanidin Extract; proanthocyanidins (GSPE); RT-PCR; Chemiluminescence Assay; Cytochrome-c Reduction Assay; Lipid Peroxidation; DNA Fragmentation; In Vitro Cell Culture System; MTT Assay

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1389201013378725

Publication date: 2001-06-01

• Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.

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• In this Subject: Nutrition & Food ,  Biotechnology ,  Pharmacology
• By this author: Joshi S.S. ;  Kuszynski C.A. ;  Bagchi D.

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