N-Glycosylation of Recombinant Pharmaceutical Glycoproteins Produced in Transgenic Plants Towards an Humanisation of Plant N-Glycans

Authors: Lerouge P.; Bardor M.; Pagny S.; Gomord V.; Faye L.

Source: Current Pharmaceutical Biotechnology, Volume 1, Number 4, December 2000 , pp. 347-354(8)

Publisher: Bentham Science Publishers

Abstract:

The number of therapeutic proteins successfully produced in plants is steadily increasing and is expected to grow even more rapidly in the future. Most therapeutic proteins are glycoproteins and N-glycosylation is often essential for their stability, folding and biological activity. Recombinant glycoproteins of mammalian origin expressed in transgenic plants largely retain their biological activity. However, plants are not ideal for production of pharmaceutical proteins because they produce molecules with glycans that are not compatible with therapeutic applications in humans. As a consequence, strategies to humanise plant N-glycans are now developed. Some of these strategies involve the retention of the recombinant glycoprotein in the endoplasmic reticulum while others are related to the inhibition of endogenous Golgi glycosyltransferases or addition of new glycosyltransferases. Data on both the N-glycosylation of therapeutic glycoproteins produced in transgenic plants and current strategies to humanise their N-glycosylation will be discussed in this review.

Keywords: Recombinant pharmaceutical glycoproteins; Transgenic plants; Protein N glycosylation; N glycans; Guys 13 monoclonal antibody

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1389201003378843

Publication date: 2000-12-01

• Current Pharmaceutical Biotechnology aims to cover all the latest and outstanding developments in Pharmaceutical Biotechnology. Each issue of the journal contains a series of timely in-depth reviews written by leaders in the field covering a range of current topics in both pre-clinical and clinical areas of Pharmaceutical Biotechnology. Current Pharmaceutical Biotechnology is an essential journal for academic, clinical, government and pharmaceutical scientists who wish to be kept informed and up-to-date with the latest and most important developments.

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• In this Subject: Nutrition & Food ,  Biotechnology ,  Pharmacology
• By this author: Lerouge P. ;  Bardor M. ;  Pagny S. ;  Gomord V. ;  Faye L.

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