A Multi-Modality Assay Platform for Ultra-High Throughput Screening
Authors: Fowler A.; Davies I.; Norey C.
Source: Current Pharmaceutical Biotechnology, Volume 1, Number 3, November 2000 , pp. 265-281(17)
Publisher: Bentham Science Publishers
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Abstract:
The demand for increased throughput during primary screening using less reagents is changing the way of drug discovery. Searching for hits using high throughput screening in 96-well format plates is being replaced by the use of higher density plates, such as 384 and 1536-well formats. The analysis of radiometric assays by scintillation counters is becoming limiting since only 12 wells can be counted at a time. Charged coupled device (CCD) camera based instruments, that image the whole plate in one exposure, speed up detection and are compatible with any microplate footprint. Researchers are also demanding a choice of detection methods, including fluorescence, luminescence and radioactivity, and require imagers suitable for all applications. LEADseeker? Homogenous Imaging System is a multi-modality platform offering imaging technology and assay toolboxes for radiometric, fluorescent and luminescent based assays. LEADseeker allows the very rapid analysis of high density formats enabling ultra-high throughput screening of a range of biological assays. Research areas that can be studied using this system include enzyme assays, receptor binding and molecular interactions.
Keywords: Multi modality assay platform; Ultra high throughput screening; Charged coupled device; Enzyme assay; Receptor binding
Language: English
Document Type: Review article
DOI: 10.2174/1389201003378933
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