Discovery Strategies for Proteomic Profiling of Airway Diseases
Abstract:Discovery proteomics is defined here as the unbiased (untargeted) differential investigation of protein abundances from biological material resulting from some biological or physical challenge. In this review, we will examine the latest approaches and strategies for discovery investigations of airway disease. We will discuss general and specific sampling strategies, sample preparation, differential separations and quantification—with special attention to posttranslational modifications that are particularly relevant to oxidative stress response of airway tissues, statistical analysis and tools, mass spectrometric identification, and finally bioinformatics tools for pathway analysis.
Keywords: Airway disease; Sypro Ruby; bioinformatics tools; chronic obstructive pulmonary disease; endogenous; gel analysis; liquid chromatography; mass spectrometry; proteomics discovery; sample fractionation; sample preparation; two dimensional gel electrophoresis
Document Type: Research Article
Publication date: July 1, 2011
- Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.