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Methods in Quantitative Proteomics: Setting iTRAQ on the Right Track

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Abstract:

Isobaric tags for absolute and relative quantification (iTRAQ) provide the quantitative proteomics community with an easy-to-use tool to examine proteome changes. Five years after the launch of the technique, has the community become familiar with iTRAQ? In this review, we propose to answer this question by looking at two of its facets. On the one hand, we enquire whether iTRAQ is used ‘optimally’; in other words, whether the community is keeping up with the methods that have been devised and the requirements that have become recognised, in quantitative proteomics. On the other hand, there is the question of whether an iTRAQ paper is written in such a way as to allow other researchers to challenge or improve the results. To tackle these two problems, we have reviewed the iTRAQ literature and gathered data about the methods; in this study, we discuss the experimental designs, the use of biological and technical replicates, the confirmation through additional experiments, the strategies to identify differential expression and supplementary information. Our conclusions aim at giving recommendations in light of our statistics.





Keywords: Enzymatic Digestion; Guidelines; ICAT; Label-Free; Replicates; SCX; SILAC; iTRAQ; mass spectrometry; methods; publication standards; quantitative proteomics; review; statistics

Document Type: Research Article

DOI: https://doi.org/10.2174/157016411794697408

Publication date: 2011-04-01

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  • Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.
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