New Proteomic Approaches to Plant Pathogenic Fungi
Authors: Garrido, Carlos; M. Cantoral, Jesus; Carbu, Maria; E. Gonzalez-Rodriguez, Victoria; Javier Fernandez-Acero, Francisco
Source: Current Proteomics, Volume 7, Number 4, December 2010 , pp. 306-315(10)
Publisher: Bentham Science Publishers
Abstract:Pathogenic fungi in plants are now one of the most serious causes of crop losses in Europe. Phytopathogenic fungi are able to infect any tissue at any stage of plant growth. Most significantly, these organisms are able to maintain their infective capacity between seasons because sources of inoculum can remain present in soils and plant debris. The value of producers' crop losses and the costs of fungicide treatments are extremely relevant economic data. The complex cycle of infection is directed by a set of genes/proteins known as pathogenicity or virulence factors. Proteomics is one of the techniques that is currently attracting much interest in the study of these organisms. At present, there is a significant increase in the number of research projects in which proteomics approaches are being used to understand the complex interactions between plants and their pathogens. This trend confirms the usefulness of these techniques for studying the biology of these pathogens, their pathogenicity or virulence factors, and for exploring new proteins that could be used as therapeutic targets. However, there are still problems to be resolved in these approaches. There is also a continuous process of improvement in many crucial stages of these proteomic studies of fungal plant pathogens - in experimental designs, in protein extraction procedures, in the separation and identification of proteins, in the analysis of results of proteome studies, and more.
This review will describe the different approaches being taken in proteomics studies of fungal plant pathogens. It will highlight the solutions being adopted to overcome the complications arising from the nature of the specific biological samples. Currently, there are no fungicides on the market that have been developed from molecular biology studies. Consequently the knowledge gained from past and present proteomic studies is still to be used in the design of fungicides, although the authors are optimistic in this respect.
Keywords: A. tereus, Botrytis cinerea; Aspergillus nidulans; Botrytis cinerea; Colletotrichum acutatum; Colletotrichum graminicola; F. oxysporum; F. verticillioides; Fusarium graminearum; M. graminicola; M. grisea; MS/MS; Magnaporthe oryzae; Mycosphaerella fijiensis; Nectria haematococca; Neurospora crassa; P. triticina; Phanerochaete chrysosporium; Proteomics; Puccinia graminis; Pulse Field Gel Electrophoresis; Restriction Fragment Length Polymorphisms; Rhizopus oryzae; Sclerotinia sclerotiorum; Southern blots; Stagonospora nodorum; Uncinola necator; Uromyces appendiculatus; Ustilago maydis; Verticillium dahliae; botrytis; carboxymethylcellulose; clesitotecio; fungi; minimal salts medium; phytopathogenic fungi
Document Type: Research Article
Publication date: 2010-12-01
- Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.