Plant Biotic Stress and Proteomics
Authors: Sergeant, Kjell; Renaut, Jenny
Source: Current Proteomics, Volume 7, Number 4, December 2010 , pp. 275-297(23)
Publisher: Bentham Science Publishers
Abstract:Being sessile, plants mainly depend on physiological and metabolic adaptations to obtain the phenotypic flexibility required to withstand the adverse biotic and abiotic growth conditions with which they are faced everyday. While the responses of plants to abiotic stresses are mainly focussed on maintaining cellular homeostasis, in the response to biotic stresses, the compatible or incompatible interaction between two different biotic entities, some very specific responses can be discerned.
The specificity of biotic stresses, generally limited to a small group of related plant species, makes that these events cannot be studied using the established model species for plant molecular research. Therefore, an overview of the techniques and tools used for the identification of proteins in biotic stress studies will be given.
The text is subdivided in two main sections, a first part focussed on an overview of the techniques that are currently used in the study of biological question using proteomics (e.g. gel- or non-gel-based techniques, posttranslational modifications or PTM) and a second part wherein some examples of biotic stress studies on plants are discussed. Because expert reviews on literature concerning the interactions of plants with the different groups of biotic stressors are part of this special issue, the emphasis is on the experimental methods and techniques used.
Keywords: 2DE gel electrophoresis; Arabidopsis thaliana; Bemisia tabaci; Biotic stress; Brassica napus; CDPKs; Fagus sylvatica; Fusarium oxysporum; Heterodera glycines; IMAC; Manduca sexta; Myzus persicae; PMF; Phytophtora citricola; Phytophtora infestans; Pissodes strobi; Ralstonia solanacearum; Sclerotinia sclerotiorum; Solanum lycopersicum; Solanum tuberosum; Syngene; Xanthomonas campestris; Xylella fastidiosa; avirulence; calcium-dependent protein kinases; effector translocator; immobilised metal affinity chromatography; induced systemic resistance; peptide mass fingerprint analysis; proteomics; sodium dodecyl sulphate; technical developments
Document Type: Research Article
Publication date: December 1, 2010
- Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.