Protein Identification and Quantification by Mass Spectrometry-Based Analysis: Applications in Plant-Pathogen Interactions Studies
Abstract:Mass spectrometry (MS) has become an essential technology for proteomics applications in biological sciences. Advances in this technique have been possible owing to improvements in MS instrumentation, new experimental strategies in sample preparation, and development of bioinformatics tools for data analyses. In recent years, complementary strategies to the classical two-dimensional gel electrophoresis approaches (2-DE) have been developed. These techniques are based on multidimensional peptide separation coupled to tandem MS (also referred as “second generation proteomics”), enabling protein expression analysis and high throughput protein identification studies. New methods such as Multidimensional Protein Identification Technology (MudPIT) and stable isotope labeling of protein/peptide samples (either by chemical, metabolic, or enzymatic methods), among others, are powerful tools for large-scale studies on characterization and expression of proteins in complex biological systems. Hence, these techniques can be very useful in the study of plant-pathogen interactions, aiding to detect and characterize both plant proteins concerned in defense reactions and pathogen proteins involved in pathogenicity and/or virulence. But these techniques have been implemented in these biological systems just recently. We will examine here how MS-based proteomics approaches are helping to better understand the multifaceted phenomena underlying plant-pathogen interactions.
Keywords: Arabidopsis thaliana; Detergent-Resistant Membranes (DRMs); Fusarium graminearum; HSP81; ICAT; Isotope-Coded Affinity Tags; Magnaporthe grisea; Mass spectrometry; MudPIT Multidimensional; NADPH oxidase; Oryza sativa; PAP; Phaseolus vulgaris; Phytophthora; Picea abies; Protein Identification Technology; Triticum aestivum; effector-triggered immunity; fibrillin; fungal xylanase; heat shock protein; hypersensitive response; iTRAQ; isobaric Tag for Relative and Absolute Quantification; omics cascade; pathogenassociated molecular patterns; peptide level proteomics; plant-pathogen interaction; plastid associated protein; protein identification; protein quantification; proteomics; second generation proteomics
Document Type: Research Article
Publication date: December 1, 2010
- Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.