Proteomics on Fixed Tissue Specimens - A Review

Authors: Reimel, Beth A.; Pan, Sheng; May, Damon H.; Shaffer, Scott A.; Goodlett, David R.; McIntosh, Martin W.; Yerian, Lisa M.; Bronner, Mary P.; Chen, Ru; Brentnall, Teresa A.

Source: Current Proteomics, Volume 6, Number 1, April 2009 , pp. 63-69(7)

Publisher: Bentham Science Publishers

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Abstract:

The vast majority of clinical tissue samples are formalin-fixed and paraffin-preserved. This type of preservation has been considered an obstacle to protein extraction from these tissues. However, these are the very tissue samples that have associated patient histories, diagnoses and outcomes - ideal samples in the quest to translate bench research into clinical applications. Thus, until recently, these valuable specimens have been unavailable for proteomic analysis.

Over the last decade, researchers have been exploring efficient methods to undo protein cross-linking caused by standard tissue fixatives and extract proteins from archived tissue specimens. These methods have been applied in different clinical proteomic studies. In this report, we attempt to review the development of these techniques, summarize the proteomic findings, and discuss the impact on future clinical proteomics.

Keywords: Proteomics; protein extraction; fixed tissue; paraffin; formalin; Hollandes; laser capture microdissection

Document Type: Research article

Publication date: 2009-04-01

More about this publication?

Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.