Author: Sun, Xue-Long

Source: Current Proteomics, Volume 5, Number 3, October 2008 , pp. 202-210(9)

Publisher: Bentham Science Publishers

Abstract:

Proteomics research typically starts by reducing the sample complexity through multidimensional separation methods based on the unique characteristics of the proteins or their peptides followed by identification and quantification. Protein-carbohydrate bindings play very important roles in cell-cell communication, cell proliferation and differentiation as well as microbial adhesion. Therefore, carbohydrates can serve as protein capturing ligand for protein purification and identification and protein-carbohydrate binding research provides important insights for basic biological research, medicine and the biotechnology as well as drug development. In the past decades, a diverse carbohydrate-based technology, termed glycotechnology, such as glyco-arrays and glyco-affinity chromatography, and glyco-affinity probe, have been developed. A guiding principle of these glycotechnologies is the display of carbohydrates onto a pertinent support or carrier such as glass slide, microplate, and microbeads as well as functional polymers and probe molecules. This review highlights recent progresses of carbohydrate-containing intelligent polymers for rapid and efficient glyco-affinity precipitation purification of carbohydrate-binding protein and the enhanced proteomics research. Specifically, two innovation methods, inverse reversible thermal-responsive glyco-affinity precipitation and magnetic assistant glyco-affinity precipitation purification of carbohydrate-binding protein for enhanced proteomics application are summarized.

Keywords: Carbohydrate; protein; glyco-affinity; stimulus responsive; polymer; precipitation; proteomics

Document Type: Research article

Publication date: 2008-10-01

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• Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.

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