Recent Advances in Controlled Immobilization of Proteins onto the Surface of the Solid Substrate and Its Possible Application to Proteomics

Authors: Nakanishi, Kazuhiro; Sakiyama, Takaharu; Kumada, Yoichi; Imamura, Koreyoshi; Imanaka, Hiroyuki

Source: Current Proteomics, Volume 5, Number 3, October 2008 , pp. 161-175(15)

Publisher: Bentham Science Publishers

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Abstract:

Proteome analysis plays a key role in the elucidation of the functions and applications for numerous proteins. For proteome analyses, various microplate- and microarray-based techniques have been developed by a number of researchers. Their intent was to immobilize proteins on the surface of a solid substrate in a site-directed manner while retaining structure and native biological function. In this review, we focus on recent advances in immobilization methodology for proteins/enzymes on a surface, including those using the affinity peptides screened by random peptide library systems. We also discuss applications of the affinity peptide-mediated immobilization method in fields related to proteome analysis, particularly our recent work concerning immunoassay and protein-protein interaction analysis.

Keywords: Controlled immobilization; site-directed immobilization; proteome analysis; affinity peptide; ELISA; proteinprotein interaction

Document Type: Research article

Publication date: 2008-10-01

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Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.