Authors: Tweedie-Cullen, Ry Y.; Wollscheid, Bernd; Livingstone-Zatchej, Magdalena; Mansuy, Isabelle M.

Source: Current Proteomics, Volume 4, Number 4, December 2007 , pp. 209-222(14)

Publisher: Bentham Science Publishers

Abstract:

Protein phosphorylation is a key post-translational modification that controls intracellular signalling in virtually all cell types. In the nervous system, it contributes to the regulation of neuronal signalling and control processes underlying synaptic plasticity and cognitive functions. However, despite its importance, knowledge about phosphoproteins and their phosphosites in the brain remains limited. A pre-requisite for unravelling brain biology and function at the molecular level, are the qualitative and quantitative analyses of protein phosphorylation and its dynamics. These analyses of the phosphoproteome require novel methodologies in addition to traditional biochemical methods. Current phosphoproteomic workflows have reached a level of maturity, which allow for their use in combination with molecular approaches, and their application to the study of higher order brain function and cognitive processes. Neuroproteomics is emerging as an essential new sub-field of the neurosciences. This review focuses on the recent advances in the application of neuroproteomics to the phosphoproteome and discusses the challenges to come.

Keywords: Phosphoproteomics; phosphorylation; phosphopeptide enrichment; stable isotope labelling; quantitative mass spectrometry; neuroproteomics; nerve cell/neuron

Document Type: Research article

Publication date: 2007-12-01

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• Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.

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