Authors: Matsumura, Nobutaka; Doi, Nobuhide; Yanagawa, Hiroshi

Source: Current Proteomics, Volume 3, Number 3, October 2006 , pp. 199-215(17)

Publisher: Bentham Science Publishers

Abstract:

Studies of protein-protein interaction networks provide a valuable framework for understanding the functional processes of living systems, because many biological processes are triggered by the interaction or binding of molecules. Display technologies are powerful tools, both for selecting and engineering polypeptides or proteins with novel functions and for analyzing protein interactions. Display technologies can be divided into two types: cell (or viral)-based display, and cell-free display. These display systems permit multiple rounds of affinity selection, and finally the amino acid sequence of the displayed protein can be determined by sequencing the corresponding DNA (or RNA). Display technologies are currently applied to select antibodies, peptides, enzymes, and biologically interacting partners. Because each display technology has various advantages and limitations, both in theory and in practice, one should adapt an appropriate method for a particular purpose. In this review, we summarize recent advances in and prospects for display technologies.

Keywords: Cell-dependent display; cell-free display; in vitro translation; antibody; peptide; unnatural amino acids; drug discovery; proteomics

Document Type: Research article

DOI: http://dx.doi.org/10.2174/157016406779475371

Affiliations: 1: Department of Biosciences and Informatics, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, Japan.

Publication date: 2006-10-01

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• Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.

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