Defining Viral Protein Interactomes Using the Yeast Two-Hybrid Assay

Authors: Diefenbach, E.; Cunningham, A. L.; Diefenbach, R. J.

Source: Current Proteomics, Volume 2, Number 3, October 2005 , pp. 225-231(7)

Publisher: Bentham Science Publishers

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Abstract:

The yeast two-hybrid assay has proved a powerful tool in identifying and characterising binary protein-protein interactions. Not only can it be used to map interacting protein domains, it can also be used to screen cDNA libraries with a desired bait to identify novel binding partners. A number of factors including ease of use, cost effectiveness and suitability for high throughput analysis have made yeast-two hybrid one of the assays of choice for defining protein-protein interaction networks or interactomes for a range of organisms. The focus of this review is on the definition of viral interactomes using the yeast two-hybrid assay and the relevance of such studies to our understanding of viral pathogenesis.

Keywords: Yeast two-hybrid assay; interactome; virus; proteomics; drug targets

Document Type: Review article

DOI: http://dx.doi.org/10.2174/157016405774641138

Affiliations: 1: Centre for Virus Research, Westmead Millennium Institute, PO Box 412, Westmead 2145 NSW, Australia.

Publication date: 2005-10-01

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Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.