Defining Viral Protein Interactomes Using the Yeast Two-Hybrid Assay

Authors: Diefenbach, E.¹; Cunningham, A. L.¹; Diefenbach, R. J.¹

Source: Current Proteomics, Volume 2, Number 3, October 2005 , pp. 225-231(7)

Publisher: Bentham Science Publishers

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Abstract:

The yeast two-hybrid assay has proved a powerful tool in identifying and characterising binary protein-protein interactions. Not only can it be used to map interacting protein domains, it can also be used to screen cDNA libraries with a desired bait to identify novel binding partners. A number of factors including ease of use, cost effectiveness and suitability for high throughput analysis have made yeast-two hybrid one of the assays of choice for defining protein-protein interaction networks or interactomes for a range of organisms. The focus of this review is on the definition of viral interactomes using the yeast two-hybrid assay and the relevance of such studies to our understanding of viral pathogenesis.

Keywords: Yeast two-hybrid assay; interactome; virus; proteomics; drug targets

Document Type: Review article

DOI: 10.2174/157016405774641138

Affiliations: 1: Centre for Virus Research, Westmead Millennium Institute, PO Box 412, Westmead 2145 NSW, Australia.

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