Identification and Characterization of Plant Allergens Using Proteomic Approaches
Authors: Law, A.1; Gupta, N.1; Louie, M.1; Poddar, R.1; Ray, A.1; Chakravarti, D. N.1
Source: Current Proteomics, Volume 2, Number 2, July 2005 , pp. 147-164(18)
Publisher: Bentham Science Publishers
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Abstract:
Rapid identification of protein allergens is important for understanding the mechanisms of pathogenesis of allergen induced diseases, and for improving diagnostic and therapeutic strategies. The most frequently used methods for characterizing protein allergens are two dimensional gel electrophoresis and immunoblot analysis. The amino acid sequences of allergens have usually been determined by Edman degradation as well as by mass spectrometry. Recent investigations have revealed a complex heterogeneity of allergen types that have varying reactivities to immunoglobulin E antibodies. This information is extremely important for the design of relevant diagnostic assays and therapeutic products. This review article attempts to evaluate the various proteomic techniques that have been used to identify and characterize protein allergens.Keywords: proteomics; plant allergens; isoallergens; d gel electrophoresis; immunoblot; maldi
Document Type: Review article
DOI: 10.2174/1570164054494172
Affiliations: 1: Keck Graduate Institute of Applied Life Sciences, 535 Watson Drive, Claremont, California 91711, USA.
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