Authors: Sinchaikul, S.; Sookkheo, B.; Topanurak, S.; Pan, Fu-Ming i.n.i.t.s..; Phutrakul, S.; Chen, Shui-Tein

Source: Current Proteomics, Volume 2, Number 2, July 2005 , pp. 109-145(37)

Publisher: Bentham Science Publishers

Abstract:

This review summarizes the current status of application of functional and structural proteomic strategies for bacteria belonging to the species Bacillus and their applications in life science research. Structural proteomics plays a crucial role for studying the relationship between structures and functions of proteins. In addition, bioinformatics is very useful for rapid search of unknown proteins including their functions, and interpretation of data in both genomics and proteomics. Expression proteome analysis based on advanced and high throughput technologyis available now is important for quantifying and analyzing protein or gene expression and understanding their biological functions. Such analysis is currently possible by the application of various multidimensional techniques, such as two-dimensional (2-D) gel electrophoresis, mass spectrometry (MS), capillary electrophoresis (CE) and other related techniques. Proteome analysis has been extensively used to study the physiology as well as molecular mechanisms of responses of Bacillus under various stress condition such as heat, cold, acid, alkali, osmotic pressure and starvation to obtain important information for comprehensive study of protein and gene functions. The applications of proteomic analysis for biomarkers and drug discovery for Bacillus sp. and the future applications of proteomics for Bacillus have been discussed.

Keywords: bacillus; proteome; functional analysis; structural analysis

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1570164054494217

Affiliations: 1: Institute of Biological Chemistry (RM707), Academia Sinica, 128 Yen Chiu Yuan Rd., Sec II, Nankang, Taipei, Taiwan 11529.

Publication date: 2005-07-01

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• Current Proteomics research in the emerging field of proteomics is growing at an extremely rapid rate. The principal aim of Current Proteomics is to publish well-timed review articles in this fast-expanding area on topics relevant and significant to the development of proteomics. Current Proteomics is an essential journal for everyone involved in proteomics and related fields in both academia and industry.

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