Functional and Structural Analysis of Bacillus Proteome

Authors: Sinchaikul, S.1; Sookkheo, B.1; Topanurak, S.1; Pan, Fu-Ming i.n.i.t.s..1; Phutrakul, S.1; Chen, Shui-Tein1

Source: Current Proteomics, Volume 2, Number 2, July 2005 , pp. 109-145(37)

Publisher: Bentham Science Publishers

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

This review summarizes the current status of application of functional and structural proteomic strategies for bacteria belonging to the species Bacillus and their applications in life science research. Structural proteomics plays a crucial role for studying the relationship between structures and functions of proteins. In addition, bioinformatics is very useful for rapid search of unknown proteins including their functions, and interpretation of data in both genomics and proteomics. Expression proteome analysis based on advanced and high throughput technologyis available now is important for quantifying and analyzing protein or gene expression and understanding their biological functions. Such analysis is currently possible by the application of various multidimensional techniques, such as two-dimensional (2-D) gel electrophoresis, mass spectrometry (MS), capillary electrophoresis (CE) and other related techniques. Proteome analysis has been extensively used to study the physiology as well as molecular mechanisms of responses of Bacillus under various stress condition such as heat, cold, acid, alkali, osmotic pressure and starvation to obtain important information for comprehensive study of protein and gene functions. The applications of proteomic analysis for biomarkers and drug discovery for Bacillus sp. and the future applications of proteomics for Bacillus have been discussed.

Keywords: bacillus; proteome; functional analysis; structural analysis

Document Type: Review article

DOI: 10.2174/1570164054494217

Affiliations: 1: Institute of Biological Chemistry (RM707), Academia Sinica, 128 Yen Chiu Yuan Rd., Sec II, Nankang, Taipei, Taiwan 11529.

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$55.10 plus tax

 

OR

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A