Proteomic Studies Using Microarrays

Authors: Tanja Feilner¹; Jurgen Kreutzberger¹; Birgit Niemann¹; Armin Kramer¹; Alexandra Possling¹; Harald Seitz¹; Birgit Kersten¹

Source: Current Proteomics, Volume 1, Number 4, December 2004 , pp. 283-295(13)

Publisher: Bentham Science Publishers

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Abstract:

Microarray technology plays an increasing role in proteomic research. We give an overview about recent developments in this technology focusing on molecular interaction studies using protein and antibody microarrays. We report about technical aspects in the development of protein microarrays and describe different surfaces and detection modes. Furthermore, we review the applications of protein microarrays in different molecular interaction studies including interactions of proteins with antibodies, proteins, DNA, small molecules and enzymes. Advantages and limitations of the microarray-based methods with other in vitro methods have been compared. We present the increasing applications of protein and antibody microarrays in basic research, diagnostics, drug discovery, and in vitro-risk assessment of nutrients.

Keywords: protein microarray; antibody microarray; protein chip; molecular interaction; enzymatic assay; serum profiling; diagnostics; drug discovery; chemical genomics

Document Type: Review article

DOI: 10.2174/1570164043152768

Affiliations: 1: Max Delbruck Center for Molecular Medicine, Department Neuroproteomics, Robert-Rossle-Str. 10, 13092 Berlin, Germany.

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