DNA Microchips: Technical and Practical Considerations

Authors: Sanchez Carbayo M.; Bornmann W.; Cordon Cardo C.

Source: Current Organic Chemistry, Volume 4, Number 9, September 2000 , pp. 945-971(27)

Publisher: Bentham Science Publishers

Buy & download fulltext article:

Price: $63.10 plus tax (Refund Policy)

Abstract:

The development of high throughput techniques, such as DNA microarrays, engages interest in many biomedical research fields. They are becoming one of the preferred methods for large-scale expression analyses. The power of this technology is that it allows the profiling of thousands of genes in one single experiment. There are two main array-based technologies: cDNA and oligonucleotide arrays hundreds to thousands of immobilized DNA probes, which are hybridized to fluorescent or radioactive complementary cDNA obtained from a target sample. Oligonucleotide chips differ in that probes are 20-25 mer selected oligonucleotides, which are bound to glass substrates and that the DNA obtained from a target sample can only be fluorescently labeled. In this review, we describe the different types of DNA-chips, the steps involved in the production of microchips, the methodological and technical aspects of microchip utilization, and their potential applications including some practical considerations utilizing clinical material.

Keywords: DNA Microchips; cDNA; Oligonucleotide arrays; DNA microarray; cDNA glass microarrays; cDNA microarrays; Image analysis

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1385272003375987

Publication date: 2000-09-01