Surface Functionalization of Nanostructured Porous Silicon by APTS: Toward the Fabrication of Electrical Biosensors of Bacterium Escherichia coli
Nanostructured porous silicon (nanoPS) basically consists in a network of silicon nanocrystals with high specific surface. Its intrinsic high surface reactivity makes nanoPS a very suitable material for the development of biosensors. In this work, the surface of nanoPS was functionalized by the use of (3-aminopropyl)triethoxysilane solutions in toluene. Escherichia coli (E. coli) antibodies were subsequently immobilized on the functionalized surfaces. Finally, fragments of this bacterium, which are specifically recognized by the antibodies, were immobilized. Moreover, devices with a metal/nanoPS/semiconductor/metal structure were fabricated aiming at the electrical biosensing of E. Coli bacterium. The experimental results showed a strong variation of the current as a function of the presence/ absence of bacterium E. Coli and surface concentration.
No Supplementary Data
Document Type: Research Article
Publication date: 2011-04-01
More about this publication?
- Current Nanoscience publishes authoritative reviews and original research reports, written by experts in the field on all the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano- structures, synthesis, properties, assembly and devices. Applications of nanoscience in biotechnology, medicine, pharmaceuticals, physics, material science and electronics are also covered. The journal is essential to all involved in nanoscience and its applied areas.