Direct Imaging of Apoptosis Process of Neural Stem Cells Exposed to Porous Silica Nanoparticles

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Abstract:

The present study was designed to examine the uptake, localization, and the cytotoxic effects of well-dispersed fluorescent porous silica nanoparticles (FITC-SiO2 NPs) in mouse neural stem cells (C 17.2, NSCs). NSCs were exposed to various concentrations of FITC-SiO2 NPs for different times and then the uptake and toxicity were assessed. Apoptotic cells were observed and analyzed by confocal microscopy and flow cytometry. Results of confocal microscopy examination revealed that silica nanoparticles were taken up into the cells. Cell viability decreased significantly as a function of both nanoparticle dosage (12- 240 μg/ml) and exposure time (12 h, 24 h 72 h). FITC-SiO2 NPs show marked toxicity at high concentration (240 μg/mL) after co-incubation for 72 h. There were clear dose- and timedependent silica-induced cytotoxicity and genotoxicity within the range of experimental concentrations. Interestingly, we have caught the whole process of NSCs apoptosis induced by FITC-SiO2 NPs. The understanding of such a mechanism may provide a scientific basis for the possible application of porous silica in drug delivery and controlled release.





Keywords: Apoptosis; Cytotoxicity; Fluorescence; Neural stem cell; Porous silica nanoparticles

Document Type: Research Article

DOI: http://dx.doi.org/10.2174/157341310791171207

Publication date: June 1, 2010

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  • Current Nanoscience publishes authoritative reviews and original research reports, written by experts in the field on all the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano- structures, synthesis, properties, assembly and devices. Applications of nanoscience in biotechnology, medicine, pharmaceuticals, physics, material science and electronics are also covered. The journal is essential to all involved in nanoscience and its applied areas.
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