Investigation of the Oligonucleotide Nanosize Delivery System with the Function of Targeting to Tumor Cells
Authors: Zhang, Zhen; Song, Xu; Wang, Baiyan; Li, Huixiang
Source: Current Nanoscience, Volume 4, Number 3, August 2008 , pp. 246-250(5)
Publisher: Bentham Science Publishers
Abstract:The effects of the tested factors including pH value, NaCl concentration, ODN concentration and charge ratio on the size and zeta potentials of nanoparticles (ODN-PLL, PLL: poly(l-lysine)s) were investigated by use of uniform design and NGR peptides were used to modify the nanoparticles, indicating that none of the tested factors was correlated with nanoparticles' size, however, a linear correlation (r = 0.8505) was found between charge ratio and zeta potential. A nanosize delivery system (ODN-PLL-NGR) with the function of targeting to tumor cells was developed by use of PLL to condense oligonucleotide into nanoparticles coated with peptide containing NGR motif. The ODN-PLL-NGR had the ability to protect ODN against nuclease degradation, and its cell uptake increased with time increment after incubation at 37°C for 40, 50 and 60 min.
Document Type: Research Article
Publication date: 2008-08-01
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