Skip to main content

Morphology of C6 Glioma Cells on a Water-Repellent Fractal Alkyl Ketene Dimer Surface

Buy Article:

$55.00 plus tax (Refund Policy)

In many in vitro cultures, cells may change their morphology, probably caused by adherence to the surface of the culture dish. Since a fractal alkyl ketene dimer (AKD) surface provides super water-repellency with a contact angle of 174° , we considered that it might provide an improved surface environment for the growth and differentiation of cells by preventing intimate adhesion. C6 glioma cells which were selected to test the effects of the fractal surface, were cultured on a conventional surface, a smooth AKD surface or a fractal AKD surface. On the conventional and smooth AKD surfaces, cells developed bipolar or multipolar shapes with enlarged cell bodies and neurite-like processes. In contrast, cells cultured on the fractal AKD surface presented fine filopodium-like processes like protoplasmic astrocytes in vivo, and higher morphological complexity was revealed by fractal analysis. Reconstruction of three-dimensional shape indicated that cells on the fractal surface were globular, whereas those on the conventional surface were rather flat. Our results suggest that C6 glioma cells on a fractal AKD surface show features of natural astrocytes with their elaborate morphology. The fractal surface thus may provide a new and natural culture environment for experimental assessment of glial structure and function.

No References
No Citations
No Supplementary Data
No Data/Media
No Metrics

Keywords: Alkyl ketene dimer (AKD); C6 glioma cell; cAMP; cytoskeleton; fractal dimension

Document Type: Research Article

Publication date: 2008-08-01

More about this publication?
  • Current Nanoscience publishes authoritative reviews and original research reports, written by experts in the field on all the most recent advances in nanoscience and nanotechnology. All aspects of the field are represented including nano- structures, synthesis, properties, assembly and devices. Applications of nanoscience in biotechnology, medicine, pharmaceuticals, physics, material science and electronics are also covered. The journal is essential to all involved in nanoscience and its applied areas.
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more