Bioluminescence Imaging of Pancreatic Islet Transplants

Authors: Xiaojuan Chen; Dixon B. Kaufman

Source: Current Medicinal Chemistry - Immunology, Endocrine & Metabolic Agents, Volume 4, Number 4, December 2004 , pp. 301-308(8)

Publisher: Bentham Science Publishers

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Abstract:

Bioluminescence imaging (BLI) modalities have been developed, refined, and used broadly in the study of small animal models of human biology and disease. Here, we review the importance of developing an in vivo imaging modality for real-time monitoring islets post transplantation. In order to advance our understanding of the pathophysiology and immunobiology of islet transplantation as they occur in living animals, islet grafts tagged with lightemitting luciferase can be implanted in a mouse islet transplantation model and assessed using in vivo BLI. Some of the preliminary data, considerations of using the system, and future applications of BLI in the field of islet transplantation research are discussed.

Keywords: bioluminescence; imaging; islet transplantation; in vivo; transgenic mice; luciferase; luciferin

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1568013043357365

Affiliations: 1: Feinberg School of Medicine, Northwestern University, Division of Transplantation, 675 N. St. Clair Street, Galter Pavilion, Suite 17-200, Chicago, IL 60611, USA.

Publication date: 2004-12-01