Sample Pretreatment and Determination of Non Steroidal Anti-Inflammatory Drugs (NSAIDs) in Pharmaceutical Formulations and Biological Samples (Blood, Plasma, Erythrocytes) by HPLC-UV-MS and mgr-HPLC

Authors: Sultan Mohamed1; Stecher Gunther1; Stoggl Wolfgang M.1; Bakry Rania1; Zaborski P.1; Huck C. W.1; El Kousy Nagla M.1; Bonn Gunther K.1

Source: Current Medicinal Chemistry, Volume 12, Number 5, March 2005 , pp. 573-588(16)

Publisher: Bentham Science Publishers

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Abstract:

The article discusses the qualitative and quantitative determination of non-steroidal antiinflammatory drugs like salicin, salicylic acid, tenoxicam, ketorolac, piroxicam, tolmetin, naproxen, flurbiprofen, diclofenac and ibuprofen by reversed phase high performance liquid chromatography (RP-HPLC) and micro-HPLC (mgr-HPLC) hyphenated with UV-absorbance and mass spectrometric detection. Both detection methods delivered calibration plots with good linearity (r2 < 0.9800), limits of detection in the low nanogram range and recovery rates between 94 and 104 %. For the analysis of biological samples such as blood, plasma and erythrocytes liquid-liquid extraction (LLE) and solid phase extraction (SPE) on the basis of new synthesized glycidylmethacrylate / divinylbenzene copolymer (GMA / DVB) particles and commercially available material on the basis of poly(divinylbenzene-co-N-vinylpyrrolidone) copolymer were investigated. Finally the use of a mgr-HPLC system with separation columns in the range of 8 cm × 200 mum I.D. for the determination of non-steroidal anti-inflammatory drugs (NSAIDs) is presented, emphasizing on the type of column and sample amount needed.

Keywords: antirheumatic; hplc; hplc; mass spectrometry; blood; plasma; erythrocytes; extraction; glycidylmetharylate/divinylbenzene copolymer

Document Type: Review article

Affiliations: 1: Institute of Analytical Chemistry and Radiochemistry, Leopold-Franzens University, Innrain 52a, 6020 Innsbruck, Austria.

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