Effect of Alpha-FMH and DPPE on Colony-forming Properties of Human Peripheral Progenitor Cells
Abstract:Endogenous histamine regulates the haematopoiesis. Histidine decarboxylase inhibitor decreases the histamine level, and its intracellular antagonist decreases the histamine effect.
The effect of histidine decarboxylase inhibitor (α-fluoromethyl histidine) and the intracellular antagonist of histamine [N'N-diethyl-2-4-(phenylmethyl) phenoxyethan-amine-HCl] was investigated on the colonyforming ability of human peripheral progenitor cells. Semi-solid culture medium was used both in the presence and in the absence of 3 U / ml erythropoietin.
α-Fluoromethyl histidine was used in the range of 50 through 150 μ Mol / ml, the concentration of N'N-diethyl- 2-4-(phenylmethyl) phenoxyethanamine-HCl was between 5 and 25 μ Mol / ml.
The number of both the erythroide and the granulocyte macrophage colony was significantly decreased in a concentration dependent manner by the presence of both N'N-diethyl-2-4-(phenylmethyl) phenoxyethanamine-HCl (in all concentrations used) and α-fluoromethyl histidine (at higher concentration). The inhibitory effect was decreased by erythropoietin.
Document Type: Review Article
Publication date: July 1, 2002
More about this publication?
- Current Medicinal Chemistry covers all the latest and outstanding developments in medicinal chemistry and rational drug design. Each issue contains a series of timely in-depth reviews written by leaders in the field covering a range of the current topics in medicinal chemistry. Current Medicinal Chemistry is an essential journal for every medicinal chemist who wishes to be kept informed and up-to-date with the latest and most important developments.