Noninvasive Genotyping of Dopamine Receptor D4 (DRD4) UsingNanograms of DNA From Substance-Dependent Patients
Authors: Boor, K.; Ronai, Z.; Nemoda, Z.; Gaszner, P.; Sasvari-Szekely, M.; Guttman, A.; Kalasz, H.
Source: Current Medicinal Chemistry, Volume 9, Number 8, April 2002 , pp. 793-797(5)
Publisher: Bentham Science Publishers
Abstract:A noninvasive DNA sampling method has been implemented collecting buccalmucosa cells by cotton wool swabs. An amount of 0.2-2 μg DNA per patient wasobtained after the phenol-extraction procedure and 0.2-2 ng DNA template wassufficient for PCR amplification of the polymorphic 48 basepair repeat region of dopaminereceptor D4 (DRD4) gene. PCR products were visualized during microfabricated electrophoretic separationby laser-induced fluorescent detection and automatic data registration. Initial data of genotyping drugdependentsubjects shows a relatively high ratio of heterozygotes, possessing either longer or shorter variantsbeside the common 4-repeat DRD4 allele.
Document Type: Review Article
Publication date: 2002-04-01
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