T Lymphocytes as Targets of Gene Transfer with Moloney-Type Retroviral Vectors

Authors: Ayuk F.A.; Zander A.R.; Fehse B.

Source: Current Gene Therapy, Volume 1, Number 4, November 2001 , pp. 325-337(13)

Publisher: Bentham Science Publishers

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Abstract:

Peripheral T lymphocytes are a target of choice for many gene therapeutic strategies. Retrovirus-mediated transduction allows genomic integration and long-term expression of transgenes in target cells. Over many years, low transduction efficiency into primary T lymphocytes has limited clinical application of existing protocols. Recently, gene transfer rates >50 percent have been achieved facilitating clinical studies. More attention is thus being focused on the ability of gene-modified cells to carry out innate as well as conferred functions in vivo and the influence of culture conditions, retroviral vector and host response thereon.

Keywords: T L ymph ocytes; Gene Transfer; TRANSGENE EXPRESSION; Gibbon ape leukemia virus

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1566523013348274

Publication date: 2001-11-01