High Throughput Single Nucleotide Polymorphism Genotyping Technology

Author: Lemieux B.

Source: Current Genomics, Volume 1, Number 4, December 2000 , pp. 301-311(11)

Publisher: Bentham Science Publishers

Key:

- Free Content

- New Content

- Subscribed Content

- Free Trial Content

Abstract:

Single nucleotide polymorphisms (SNP) are the most abundant of all DNA polymorphisms. The rapid progress of genome projects presents a unique opportunity to genetic researchers if reliable high throughput methods can be developed for SNP typing. In this review we will discuss the broad variety of SNP typing formats and discuss their relative merits for direct and indirect association studies. We will also mention some of the possible technical advances which may impact SNP typing in the next few years.

Keywords: High Throughput Single Nucleotide Polymorphism Gen; Single nucleotide polymorhisms SNP; PCR; short sequence; DNA polymerase; DNA replication; DNA Ligase Based Assay; Invasive Probe Assays; Microsequencing; Gene Chip Assays