Free Content Label-Free Cell-Based Assays for GPCR Screening

Authors: Fang, Ye; Frutos, Anthony G.; Verklereen, Ronald

Source: Combinatorial Chemistry & High Throughput Screening, Volume 11, Number 5, June 2008 , pp. 357-369(13)

Publisher: Bentham Science Publishers

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Abstract:

G protein-coupled receptors (GPCRs) have been proven to be the largest family of druggable targets in the human genome. Given the importance of GPCRs as drug targets and the de-orphanization of novel targets, GPCRs are likely to remain the frequent targets of many drug discovery programs. With recent advances in instrumentation and understanding of cellular mechanisms for the signals measured, biosensor-centered label-free cell assay technologies become a very active area for GPCR screening. This article reviews the principles and potential of current label-free cell assay technologies in GPCR drug discovery.

Keywords: G protein-coupled receptor; optical biosensor; resonant waveguide grating biosensor; electrical biosensor; dynamic mass redistribution; impedance; high throughput screening

Document Type: Research article

DOI: http://dx.doi.org/10.2174/138620708784534789

Affiliations: 1: Biochemical Technologies,Science and Technology Division, Corning Incorporated, Sullivan Park,Corning, NY 14831, USA.

Publication date: 2008-06-01

More about this publication?
  • Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.
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