Automated Acquisition of Aptamer Sequences

Authors: Cox J.Colin; Rajendran M.; Riedel T.; Davidson E.A.; Sooter L.J.; Bayer T.S.; Schmitz-Brown M.; Ellington A.D.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 5, Number 4, June 2002 , pp. 289-299(11)

Publisher: Bentham Science Publishers

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Abstract:

While the in vitro selection of nucleic acid binding species (aptamers) requires numerous liquidhandling steps, these steps are relatively straightforward and the overall process is therefore amenable to automation. Here we demonstrate that automated selection techniques are capable of generating aptamers against a number of diverse protein targets. Automated selection techniques can be integrated with automated analytical methods, including sequencing, determination of binding constants, and structural analysis. The methods that have so far been developed can be further multiplexed, and it should soon be possible to attempt the selection of aptamers against organismal proteomes or metabolomes.

Keywords: Automated Acquisition; Aptamer Sequences; Protein Targets

Language: English

Document Type: Review article

DOI: 10.2174/1386207023330291

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