Automated Acquisition of Aptamer Sequences

Authors: Cox J.Colin; Rajendran M.; Riedel T.; Davidson E.A.; Sooter L.J.; Bayer T.S.; Schmitz-Brown M.; Ellington A.D.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 5, Number 4, June 2002 , pp. 289-299(11)

Publisher: Bentham Science Publishers

Abstract:

While the in vitro selection of nucleic acid binding species (aptamers) requires numerous liquidhandling steps, these steps are relatively straightforward and the overall process is therefore amenable to automation. Here we demonstrate that automated selection techniques are capable of generating aptamers against a number of diverse protein targets. Automated selection techniques can be integrated with automated analytical methods, including sequencing, determination of binding constants, and structural analysis. The methods that have so far been developed can be further multiplexed, and it should soon be possible to attempt the selection of aptamers against organismal proteomes or metabolomes.

Keywords: Automated Acquisition; Aptamer Sequences; Protein Targets

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1386207023330291

Publication date: 2002-06-01

• Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.

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• By this author: Cox J.Colin ;  Rajendran M. ;  Riedel T. ;  Davidson E.A. ;  Sooter L.J. ;  Bayer T.S. ;  Schmitz-Brown M. ;  Ellington A.D.

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