Parallel Synthesis of PNA-Peptide Conjugate Libraries

Authors: Awasthi S.Kumar; Nielsen P.E.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 5, Number 3, May 2002 , pp. 253-259(7)

Publisher: Bentham Science Publishers

Key:

- Free Content

- New Content

- Subscribed Content

- Free Trial Content

Abstract:

An optimized semi-automatic protocol for parallel synthesis of up to 96 peptide nucleic acids (PNA) or PNA-peptide conjugates using Boc-protection strategy has been developed using a robotic system. The approach is illustrated by synthesizing PNA and PNA-peptide libraries varying between 15 and 27 “amino acid” units. The peptides (NLS (nuclear localization signal) or Tat-peptide) were attached to N-terminus of the PNA. The method was found to be far superior to that based on the SPOT / Fmoc protocol by which PNA oligomers are synthesized on a modified cellulose membrane. On a 0.5 micromole scale the method typically yielded 2 mg product of 90% purity by HPLC / MALDI-TOF analysis. This approach is suitable for screening of a large number of PNA and / or peptide sequences for biochemical and biological studies.

Keywords: pna libraries; pna-peptide libraries; boc-protection strategy

Language: English

Document Type: Review article

DOI: 10.2174/1386207024607266

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$55.10 plus tax Refund Policy