The Cloning of Human Genes Using cDNA Phage Display and Small-Molecule Chemical Probes

Authors: Savinov S.N.; Austin D.J.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 4, Number 6, September 2001 , pp. 593-597(6)

Publisher: Bentham Science Publishers

Abstract:

The cloning of genes based on protein function has become a powerful tool for protein discovery and should play an important role in proteomics in general. We have recently reported a technique for the functional identification of protein targets by combining traditional affinity chromatography with cDNA phage display. This procedure, referred to as display cloning, directly couples biologically active natural products to the gene of their protein cellular target. We now report the cloning of a human gene, the a domain of F1 ATP synthase, using a synthetic scaffold molecule which serves as a prototype for a diverse chemical library. The ability to select genes from cDNA libraries using probes from combinatorial libraries would greatly increase the number of small molecule / protein interactions that can be identified. This method might prove valuable in furthering our understanding of biology and its application toward drug development.

Keywords: cDNA Phage Display; proteomics

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1386207013330922

Publication date: 2001-09-01

• Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.

• In this: publication
• By this: publisher
• In this Subject: Pharmacology
• By this author: Savinov S.N. ;  Austin D.J.

Website © Publishing Technology. Article copyright remains with the publisher, society or author(s) as specified within the article.

• Terms and conditions
• Privacy policy
• Information for advertisers