Recognition of Peptides by Antibodies and Investigations of Affinity Using Biosensor Technology

Authors: Regenmortel M.H.V.Van; Choulier L.

Source: Combinatorial Chemistry & High Throughput Screening, Volume 4, Number 5, August 2001 , pp. 385-395(11)

Publisher: Bentham Science Publishers

Abstract:

The study of peptide-antibody interactions has many applications in biology and medicine. Synthetic peptides corresponding to single protein epitopes are used instead of intact proteins as reagents for the diagnosis of viral and autoimmune diseases. Furthermore, antibodies raised against peptides are useful reagents for isolating and characterizing gene products. In this review, methods for analysing the molecular basis of peptide-antibody interactions are described, such as amino acid replacement studies, X-ray crystallography of peptide-antibody complexes and biosensor technology based on surface plasmon resonance. The importance of peptide conformation in antibody recognition is discussed, and the antigenic reactivity of epitopes in synthetic peptides and in cognate, intact proteins is compared.

Keywords: Biosensor Technology; peptide antibody interactions; autoimmune diseases; gliadins; glutenins; surrogate protein epitopes; mimotopes; peptide antigenicity; foot-and-mouthdisease virus fmdv; tobacco mosaic virus protein; cognate proteins

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/1386207013330940

Publication date: 2001-08-01

• Combinatorial Chemistry & High Throughput Screening publishes full length original research articles and reviews describing various topics in combinatorial chemistry (e.g. small molecules, peptide, nucleic acid or phage display libraries) and/or high throughput screening (e.g. developmental, practical or theoretical). Ancillary subjects of key importance, such as robotics and informatics, will also be covered by the journal. In these respective subject areas, Combinatorial Chemistry & High Throughput Screening is intended to function as the most comprehensive and up-to-date medium available. The journal should be of value to individuals engaged in the process of drug discoveryand development, in the settings of industry, academia or government.

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